Author ORCID Identifier
https://orcid.org/0000-0001-7251-8739
Date of Award
17-8-2025
Document Type
Thesis
School
School of Chemical & Biotechnology
Programme
Ph.D.-Doctoral of Philosophy
First Advisor
Dr.Shanker Jha
Second Advisor
Dr.Prakash Shankaran
Keywords
Class II Transactivator, Interactome Analysis, Ciita Interactors, Molecular Docking, Neuroinflammation
Abstract
Inflammation plays a significant role in maintaining homeostasis. Neuroinflammation is a chronic inflammatory response in the central nervous system (CNS), leading to brain or spinal cord dysfunction. To promote mental health, it is crucial to understand the regulation of neuroinflammation. Class II transactivator (CIITA) is a master-regulator of the major histocompatibility complex II (MHCII).
Modulation of CIITA has been suggested as a promising intervention for inflammatory conditions, including neurological disorders. However, there is limited knowledge about the role of CIITA and its interactors in inflammation. This study aimed to understand the role of CIITA in neuroinflammation and neurological disorders using in-silico tools.
Here, we 1) constructed a phylogenetic tree for CIITA, 2) predicted its structures using computational tools, 3) analyzed its interactome with neuroinflammatory genes, 4) studied differential expression of CIITA and its interactors in the zebrafish brain following peripherally induced inflammation and, 5) performed in-silico screening to identify small molecules that could bind to the predicted structure of CIITA. Phylogenetic analysis revealed that the closest orthologs for human CIITA (hCIITA) are in monkeys, orangutans, mice, rats, frogs, and zebrafish.
The interactome analysis predicted that CIITA might primarily interact with the IL4/IL13 and hippo signaling pathways during neuroinflammation. LPS-induced inflammation in zebrafish suggested a role for CIITA in chronic inflammation in the brain.
Finally, based on our in-silico drug screening, we propose five molecules (ZINC5154833, F5254-0161, Arteannuin B, Creatinine, and Natural2) that could target CIITA. These results will help us understand the possible molecular pathways by which CIITA could regulate neuroinflammation and brain homeostasis.
Recommended Citation
N, Kishore Mr, "In Silico Investigations of Class II Trans Activator (CIITA): Its Plausible Implications in Neuroinflammation and Neurological Disorders" (2025). Theses and Dissertations. 48.
https://knowledgeconnect.sastra.edu/theses/48