Author ORCID Identifier

0000-0002-9324-6442

Author Linked-In Account

linkedin.com/in/lnhr

Date of Award

17-8-2025

Document Type

Thesis

School

School of Chemical & Biotechnology

Programme

Ph.D.-Doctoral of Philosophy

First Advisor

Dr.M.Meenakshi Sundaram

Keywords

Adsorption, Quercetin, Magnetic Molecularly Imprinted Polymer, Rosa Damascene Petal, Allium Cepa Peel

Abstract

A highly selective Magnetic Molecularly Imprinted Polymer (MMIP) was developed for the detection and purification of Quercetin. The Density Functional Theory was utilized for the selection of suitable monomers based on binding energy interaction between quercetin and monomers (Acrylamide, Acrylonitrile, Methacrylic Acid). From computational studies, Acrylamide was selected as a suitable monomer for the synthesis of MMIP. Under ultrasound irradiation both quercetin and naringin imprinted materials MMIP-Q and MMIP-N were fabricated.

Compared to ultrasound-assisted synthesis, Microwave Synthesis provides uniform heat transfer throughout the reaction solution, which enables uniformity in the size of synthesised material, increases the yield of material and reduces the reaction time. Therefore, Microwave-assisted Quercetin imprinted Magnetic Molecularly Imprinted Polymer (MMIP-Q (MW)) was synthesised. The maximum adsorption capacity, adsorption isotherm and adsorption kinetic studies were analysed for MMIP-Q, MMIP-N and MMIP-Q (MW).

The sorbents developed were coupled with HPLC-UV for the detection of quercetin from Rose damascene petals. In order to purify quercetin, Supercritical fluid extraction was performed for the extraction of quercetin from rose petals. Hot water extraction and liquid-liquid extraction were performed for extraction of quercetin from onion peel. After the extraction process, purification has been performed with developed sorbents.

From all the above synthesised materials, MMIP-Q (MW) exhibits high stability, highest recovery of 98% from rose petal extract and 31.2% from onion peel extract. The purity of recovered quercetin was identified to be 55.1% from rose petals and 52.7% from onion peel. In addition to quercetin, rose petal extract was detected with compounds such as kaempferol-3-O-rhamnoside, quercetin-3-O-glucoside and quercetin-3-O-rhamnoside, whereas purified onion peel extract was detected with quercetin-3-O-glucoside.

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